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anti emd rabbit polyclonal  (Proteintech)


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    Structured Review

    Proteintech anti emd rabbit polyclonal
    Anti Emd Rabbit Polyclonal, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+emerin+polyclonal+antibody/Emerin+Antibody/pmc12393879-50-2-6
    Average 94 stars, based on 45 article reviews
    anti emd rabbit polyclonal - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Western Blot:

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624).. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432) antibodies. qPCR Total RNA from tumors and normal breast tissue controls were purchased from Origene Technologies, Inc.Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432)..

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells. .. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624). .. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invi

    Expressing:

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624).. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432) antibodies. qPCR Total RNA from tumors and normal breast tissue controls were purchased from Origene Technologies, Inc.Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432)..

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells. .. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624). .. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invi

    Fluorescence:

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624).. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432) antibodies. qPCR Total RNA from tumors and normal breast tissue controls were purchased from Origene Technologies, Inc.Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432)..

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells. .. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624). .. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invi

    Mutagenesis:

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624).. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432) antibodies. qPCR Total RNA from tumors and normal breast tissue controls were purchased from Origene Technologies, Inc.Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432)..

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells. .. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624). .. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invi

    Injection:

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624).. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432) antibodies. qPCR Total RNA from tumors and normal breast tissue controls were purchased from Origene Technologies, Inc.Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432)..

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells. .. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624). .. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invi

    Control:

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624).. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432) antibodies. qPCR Total RNA from tumors and normal breast tissue controls were purchased from Origene Technologies, Inc.Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432)..

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells. .. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624). .. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invi

    Plasmid Preparation:

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624).. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432) antibodies. qPCR Total RNA from tumors and normal breast tissue controls were purchased from Origene Technologies, Inc.Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432)..

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells. .. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624). .. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invi

    Electroporation:

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624).. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432) antibodies. qPCR Total RNA from tumors and normal breast tissue controls were purchased from Origene Technologies, Inc.Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432)..

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells. .. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624). .. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invi

    Stable Transfection:

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624).. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432) antibodies. qPCR Total RNA from tumors and normal breast tissue controls were purchased from Origene Technologies, Inc.Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432)..

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells. .. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624). .. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invi

    Standard Deviation:

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624).. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432) antibodies. qPCR Total RNA from tumors and normal breast tissue controls were purchased from Origene Technologies, Inc.Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432)..

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells. .. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624). .. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invi

    Binding Assay:

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624).. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432) antibodies. qPCR Total RNA from tumors and normal breast tissue controls were purchased from Origene Technologies, Inc.Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432)..

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells. .. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624). .. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invi

    Disruption:

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624).. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432) antibodies. qPCR Total RNA from tumors and normal breast tissue controls were purchased from Origene Technologies, Inc.Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432)..

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells. .. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624). .. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invi

    Wound Healing Assay:

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624).. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432) antibodies. qPCR Total RNA from tumors and normal breast tissue controls were purchased from Origene Technologies, Inc.Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432)..

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells. .. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624). .. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invi

    Transferring:

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624).. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432) antibodies. qPCR Total RNA from tumors and normal breast tissue controls were purchased from Origene Technologies, Inc.Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432)..

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells. .. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624). .. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invi

    Migration:

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624).. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432) antibodies. qPCR Total RNA from tumors and normal breast tissue controls were purchased from Origene Technologies, Inc.Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432)..

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells. .. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624). .. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invi

    Blocking Assay:

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells.. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624).. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432) antibodies. qPCR Total RNA from tumors and normal breast tissue controls were purchased from Origene Technologies, Inc.Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invitrogen, Carlsbad, CA, cat#: 31462) and mouse (1:10,000 dilution; Invitrogen, cat#: 31432)..

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis
    Article Snippet: cells expressing GFP-emerin or each GFP-emerin mutant were normalized to MDA-231 cells. .. Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624). .. Secondary antibodies included horseradish peroxidase-conjugated rabbit (1:10,000 dilution; Invi



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    Image Search Results


    (A) Western Blot analysis of emerin in primary mammary epithelial cells, MCF10A cells, MDA-231 cells and MDA-157 cells. (B) Western Blot quantification of emerin expression for each cell line. Emerin expression was normalized to tubulin and primary mammary epithelial cells. Error bars represent standard error. (n=3) ****p-value < 0.0001, unpaired t-test. (C) Nuclear area for MCF10A (n=116, blue), MDA-231 (n=237, green) and MDA-157 (n=342, red) cells. Error bars represent standard error. ****p-value < 0.0001, unpaired t-test (D) Representative DAPI (blue) images of MCF10A, MDA-231, and MDA-157 cells. Scale bars: 100 μm. (E) Nuclear circularity for MCF10A (n=75, blue), MDA-231 (n=301, green), and MDA-157 (n=237, red) cells. Error bars represent standard error. ****p-value < 0.0001, unpaired t-test

    Journal: Molecular cancer research : MCR

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis

    doi: 10.1158/1541-7786.MCR-20-0413

    Figure Lengend Snippet: (A) Western Blot analysis of emerin in primary mammary epithelial cells, MCF10A cells, MDA-231 cells and MDA-157 cells. (B) Western Blot quantification of emerin expression for each cell line. Emerin expression was normalized to tubulin and primary mammary epithelial cells. Error bars represent standard error. (n=3) ****p-value < 0.0001, unpaired t-test. (C) Nuclear area for MCF10A (n=116, blue), MDA-231 (n=237, green) and MDA-157 (n=342, red) cells. Error bars represent standard error. ****p-value < 0.0001, unpaired t-test (D) Representative DAPI (blue) images of MCF10A, MDA-231, and MDA-157 cells. Scale bars: 100 μm. (E) Nuclear circularity for MCF10A (n=75, blue), MDA-231 (n=301, green), and MDA-157 (n=237, red) cells. Error bars represent standard error. ****p-value < 0.0001, unpaired t-test

    Article Snippet: Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624).

    Techniques: Western Blot, Expressing

    (A) Fluorescence intensity of the primary tumor was measured weekly for eight weeks. (B) Primary tumor size after excision at week eight. n=38 for iRFP713, n=30 for GFP-emerin, n=28 for each GFP-emerin mutant. Error bars represent standard error. *p-value = 0.0004, **p-value = 0.0050, ***p-value = 0.0006, ****p-value= 0.0266, one-way ANOVA. C) Volume of excised tumors at eight weeks post-injection with MDA-231 cells expressing iRFP713 (n=38), and GFP-emerin (n=30), GFP-S54F (n=28), GFP-M196 (n=28), GFP-M45A (n=28), or GFP-M151 (n=28) cells. Error bars represent standard error. *p-value = 0.0005, **p-value = 0.0015, ***p-value = 0.0239, one-way ANOVA (D) Representative fluorescent images of excised primary tumors at eight weeks post-injection. Heat-map shows iRFP713 fluorescence intensity. Scale bars: 1 cm (E) Fluorescence intensity of lung metastasis at eight weeks post-injection. Data was normalized to control lungs with no metastasis. n=38 for iRFP713 alone, n=30 for GFP-emerin, n=28 for each GFP-emerin mutant. Error bars represent standard error. *p-value = 0.0027, **p-value = 0.0040, ***p-value = 0.0034, ****p-value = 0.0156, *****p-value = 0.0107, one-way ANOVA. (F) Lung metastasis fluorescence intensity from mice whose primary tumors were greater than 50,000 FU is shown. Data was normalized to control lungs with no metastasis. n=28 for iRFP713, n=12 for GFP-emerin, n=20 for GFP-S54F, n=16 for GFP-M196, n=24 for GFP-M45A, n=28 for GFP-M151. Error bars represent standard error. *p-value = 0.0023, **p-value = 0.0035, ***p-value = 0.0331, ****p-value = 0.0136, *****p-value = 0.0006, one-way ANOVA. (G) Representative fluorescent images of excised lungs at eight weeks post-injection. Heat-map shows iRFP713 fluorescent intensity.

    Journal: Molecular cancer research : MCR

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis

    doi: 10.1158/1541-7786.MCR-20-0413

    Figure Lengend Snippet: (A) Fluorescence intensity of the primary tumor was measured weekly for eight weeks. (B) Primary tumor size after excision at week eight. n=38 for iRFP713, n=30 for GFP-emerin, n=28 for each GFP-emerin mutant. Error bars represent standard error. *p-value = 0.0004, **p-value = 0.0050, ***p-value = 0.0006, ****p-value= 0.0266, one-way ANOVA. C) Volume of excised tumors at eight weeks post-injection with MDA-231 cells expressing iRFP713 (n=38), and GFP-emerin (n=30), GFP-S54F (n=28), GFP-M196 (n=28), GFP-M45A (n=28), or GFP-M151 (n=28) cells. Error bars represent standard error. *p-value = 0.0005, **p-value = 0.0015, ***p-value = 0.0239, one-way ANOVA (D) Representative fluorescent images of excised primary tumors at eight weeks post-injection. Heat-map shows iRFP713 fluorescence intensity. Scale bars: 1 cm (E) Fluorescence intensity of lung metastasis at eight weeks post-injection. Data was normalized to control lungs with no metastasis. n=38 for iRFP713 alone, n=30 for GFP-emerin, n=28 for each GFP-emerin mutant. Error bars represent standard error. *p-value = 0.0027, **p-value = 0.0040, ***p-value = 0.0034, ****p-value = 0.0156, *****p-value = 0.0107, one-way ANOVA. (F) Lung metastasis fluorescence intensity from mice whose primary tumors were greater than 50,000 FU is shown. Data was normalized to control lungs with no metastasis. n=28 for iRFP713, n=12 for GFP-emerin, n=20 for GFP-S54F, n=16 for GFP-M196, n=24 for GFP-M45A, n=28 for GFP-M151. Error bars represent standard error. *p-value = 0.0023, **p-value = 0.0035, ***p-value = 0.0331, ****p-value = 0.0136, *****p-value = 0.0006, one-way ANOVA. (G) Representative fluorescent images of excised lungs at eight weeks post-injection. Heat-map shows iRFP713 fluorescent intensity.

    Article Snippet: Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624).

    Techniques: Fluorescence, Mutagenesis, Injection, Expressing, Control

    (A) Nuclear area of MDA-231 cells (n=237, black), MDA-231 cells with no plasmid, but electroporated (electroporation control; n=195, red), and MDA-231 cells expressing GFP (n=53, orange) or GFP-emerin (n=157, blue). Error bars represent standard error. ****p-value < 0.0001, unpaired t-test (B) Representative DAPI (blue) and GFP-emerin (green) images of MDA-231 cells. Scale bar: 50 μm. (C) Nuclear area of MCF10A cells (n=65, black), MCF10A cells with no plasmid, but electroporated (electroporation control; n=45, red), and MCF10A cells expressing GFP (n=90, orange) or GFP-emerin (n=76, blue). Error bars represent standard error. All comparisons had p-values > 0.05. (D) Representative DAPI (blue) and GFP-emerin (green) images of MCF10A cells. Scale bar: 50 μm.

    Journal: Molecular cancer research : MCR

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis

    doi: 10.1158/1541-7786.MCR-20-0413

    Figure Lengend Snippet: (A) Nuclear area of MDA-231 cells (n=237, black), MDA-231 cells with no plasmid, but electroporated (electroporation control; n=195, red), and MDA-231 cells expressing GFP (n=53, orange) or GFP-emerin (n=157, blue). Error bars represent standard error. ****p-value < 0.0001, unpaired t-test (B) Representative DAPI (blue) and GFP-emerin (green) images of MDA-231 cells. Scale bar: 50 μm. (C) Nuclear area of MCF10A cells (n=65, black), MCF10A cells with no plasmid, but electroporated (electroporation control; n=45, red), and MCF10A cells expressing GFP (n=90, orange) or GFP-emerin (n=76, blue). Error bars represent standard error. All comparisons had p-values > 0.05. (D) Representative DAPI (blue) and GFP-emerin (green) images of MCF10A cells. Scale bar: 50 μm.

    Article Snippet: Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624).

    Techniques: Plasmid Preparation, Electroporation, Control, Expressing

    (A) Western blot of MCF10A cells, MDA-231 cells and MDA-231 cells stably expressing GFP-emerin or each GFP-emerin mutant. (B) Endogenous emerin, GFP-emerin and total emerin protein expression was normalized to gamma-tubulin and wildtype emerin in MDA-231 cells. Error bars represent standard deviation. (C) Panel showing known emerin-binding proteins and their disruption by the indicated emerin mutant protein. +, indicates emerin mutant binds to specific binding partner; -, indicates emerin mutant disrupts binding to the protein [58]. Disruptions are highlighted in yellow for clarity. (D) Nuclear area of MDA-231 cells (n=237, black), MDA-231 cells with no plasmid, but electroporated (electroporation control; n=195, black), and MDA-231 cells expressing GFP (n=53, orange), GFP-emerin (n=157, blue), GFP-M45A (n=142, green), GFP-S54F (n=145, purple), GFP-M151 (n=155, yellow), GFP-M24 (n=158, pink) or GFP-M196 (n=149, cyan). Error bars represent standard error. ****p-value < 0.0001, one-way ANOVA. (E) Representative DAPI (blue) and GFP (green) images for GFP-emerin and each GFP-emerin mutant analyzed in D. Scale bars: 50 μm. (F) Nuclear volume of MCF10A cells (n=25, red), MDA-231 cells (n=29, black), and MDA-231 cells expressing GFP-emerin (n=32, blue), GFP-M45A (n=22, green), GFP-S54F (n=28, purple), GFP-M151 (n=26, yellow), and GFP-M196 (n=19, cyan). Error bars represent standard error. **p-value = 0.0030, *p-value = 0.041, one-way ANOVA. (G) Representative DAPI (blue), GFP (green), and merged images for MCF10A cells, MDA-231 cells and MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant analyzed in F. Scale bars: 10 μm. (H) Nuclear circularity of MDA-231 cells (n=55, black) and MDA-231 cells expressing GFP-emerin (n=52, blue), GFP-M45A (n=53), green), GFP-S54F (n=50, purple), GFP-M151 (n=49, yellow), GFP-M24 (n=44, pink), or GFP-M196 (n=59, cyan). Error bars represent standard error. *p-value = 0.0035, **p-value = 0.0005, ***p-value = 0.0225, ****p-value < 0.0001, one-way ANOVA (I) Ratio of nuclear to cytoplasmic area in MDA-231 cells (n=61, black) and MDA-231 cells expressing GFP-emerin (n=63, blue), GFP-M45A (n=45, green), GFP-S54F (n=65, purple), GFP-M151 (n=55, yellow) or GFP-M196 (n=54, cyan). Error bars represent standard error. *p-value = 0.051, **p-value = 0.035, ****p-value < 0.0001, one-way ANOVA. (J) Nuclear area of MCF10A cells (n=65, black), MCF10A cells with no plasmid, but electroporated (electroporation control; n=45, red), and MCF10A cells expressing GFP (n=90, orange), GFP-emerin (n=76, blue), GFP-M45A (n=90, green), GFP-S54F (n=91, purple), GFP-M151 (n=72, yellow), GFP-M24 (n=72, pink) or GFP-M196 (n=72, cyan). Error bars represent standard error. **p-value = 0.0101, one-way ANOVA.

    Journal: Molecular cancer research : MCR

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis

    doi: 10.1158/1541-7786.MCR-20-0413

    Figure Lengend Snippet: (A) Western blot of MCF10A cells, MDA-231 cells and MDA-231 cells stably expressing GFP-emerin or each GFP-emerin mutant. (B) Endogenous emerin, GFP-emerin and total emerin protein expression was normalized to gamma-tubulin and wildtype emerin in MDA-231 cells. Error bars represent standard deviation. (C) Panel showing known emerin-binding proteins and their disruption by the indicated emerin mutant protein. +, indicates emerin mutant binds to specific binding partner; -, indicates emerin mutant disrupts binding to the protein [58]. Disruptions are highlighted in yellow for clarity. (D) Nuclear area of MDA-231 cells (n=237, black), MDA-231 cells with no plasmid, but electroporated (electroporation control; n=195, black), and MDA-231 cells expressing GFP (n=53, orange), GFP-emerin (n=157, blue), GFP-M45A (n=142, green), GFP-S54F (n=145, purple), GFP-M151 (n=155, yellow), GFP-M24 (n=158, pink) or GFP-M196 (n=149, cyan). Error bars represent standard error. ****p-value < 0.0001, one-way ANOVA. (E) Representative DAPI (blue) and GFP (green) images for GFP-emerin and each GFP-emerin mutant analyzed in D. Scale bars: 50 μm. (F) Nuclear volume of MCF10A cells (n=25, red), MDA-231 cells (n=29, black), and MDA-231 cells expressing GFP-emerin (n=32, blue), GFP-M45A (n=22, green), GFP-S54F (n=28, purple), GFP-M151 (n=26, yellow), and GFP-M196 (n=19, cyan). Error bars represent standard error. **p-value = 0.0030, *p-value = 0.041, one-way ANOVA. (G) Representative DAPI (blue), GFP (green), and merged images for MCF10A cells, MDA-231 cells and MDA-231 cells expressing GFP-emerin or each GFP-emerin mutant analyzed in F. Scale bars: 10 μm. (H) Nuclear circularity of MDA-231 cells (n=55, black) and MDA-231 cells expressing GFP-emerin (n=52, blue), GFP-M45A (n=53), green), GFP-S54F (n=50, purple), GFP-M151 (n=49, yellow), GFP-M24 (n=44, pink), or GFP-M196 (n=59, cyan). Error bars represent standard error. *p-value = 0.0035, **p-value = 0.0005, ***p-value = 0.0225, ****p-value < 0.0001, one-way ANOVA (I) Ratio of nuclear to cytoplasmic area in MDA-231 cells (n=61, black) and MDA-231 cells expressing GFP-emerin (n=63, blue), GFP-M45A (n=45, green), GFP-S54F (n=65, purple), GFP-M151 (n=55, yellow) or GFP-M196 (n=54, cyan). Error bars represent standard error. *p-value = 0.051, **p-value = 0.035, ****p-value < 0.0001, one-way ANOVA. (J) Nuclear area of MCF10A cells (n=65, black), MCF10A cells with no plasmid, but electroporated (electroporation control; n=45, red), and MCF10A cells expressing GFP (n=90, orange), GFP-emerin (n=76, blue), GFP-M45A (n=90, green), GFP-S54F (n=91, purple), GFP-M151 (n=72, yellow), GFP-M24 (n=72, pink) or GFP-M196 (n=72, cyan). Error bars represent standard error. **p-value = 0.0101, one-way ANOVA.

    Article Snippet: Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624).

    Techniques: Western Blot, Stable Transfection, Expressing, Mutagenesis, Standard Deviation, Binding Assay, Disruption, Plasmid Preparation, Electroporation, Control

    (A) Western blot quantification of H3K9me2, H3K9me3, and H3K27me3 expression in MDA-231 cells, and MDA-231 cells expressing GFP-emerin, GFP-M45A, GFP-S54F, GFP-M151, and GFP-M196. Expression was normalized to gamma-tubulin and MDA-231 cells. Error bars represent standard error. All comparisons had p-value > 0.05. (B) Representative Western blot of MDA-231 cells and MDA-231 cells expressing GFP-emerin, GFP-M45A, GFP-S54F, GFP-M151, and GFP-M196. (C) The number of cells migrating through 3.0 μm transwell pores is shown for MCF10A cells, MDA-231 cells, and MDA-231 cells expressing GFP, GFP-emerin, GFP-M45A, GFP-S54F, GFP-M151, GFP-M24 or GFP-M196. Error bars represent standard deviation. ****p-value < 0.0001, one-way ANOVA (D) Representative DAPI (blue) images for each of the cell lines analyzed in C. Scale bars: 400 μm. (E) The number of cells invading through 8.0 μm transwell pores with a Matrigel® coating was measured for MCF10A cells, MDA-231 cells, and MDA-231 cells expressing GFP-emerin, GFP-M45A, GFP-S54F, GFP-M151, or GFP-M196. Error bars represent standard deviation. *p-value = 0.0009, **p-value = 0.0005, ****p-value < 0.0001, one-way ANOVA (F) Representative DAPI (blue) images used for E. Scale Bars: 400 μm. (G) Scratch-wound healing assay. MCF10A cells, MDA-231 cells, and MDA-231 cells expressing GFP-emerin, GFP-M45A, GFP-S54F, GFP-M151, or GFP-M196 were plated, scratched with a pipette tip, and migration of cells into the wound area was monitored for 2, 4, 12 and 24 hours. Percent healed (%) refers to the ability of cells to migrate into the wound area. Error bars represent standard deviation. (H) Representative phase images for each cell line analyzed in G. Scale bars: 400 μm.

    Journal: Molecular cancer research : MCR

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis

    doi: 10.1158/1541-7786.MCR-20-0413

    Figure Lengend Snippet: (A) Western blot quantification of H3K9me2, H3K9me3, and H3K27me3 expression in MDA-231 cells, and MDA-231 cells expressing GFP-emerin, GFP-M45A, GFP-S54F, GFP-M151, and GFP-M196. Expression was normalized to gamma-tubulin and MDA-231 cells. Error bars represent standard error. All comparisons had p-value > 0.05. (B) Representative Western blot of MDA-231 cells and MDA-231 cells expressing GFP-emerin, GFP-M45A, GFP-S54F, GFP-M151, and GFP-M196. (C) The number of cells migrating through 3.0 μm transwell pores is shown for MCF10A cells, MDA-231 cells, and MDA-231 cells expressing GFP, GFP-emerin, GFP-M45A, GFP-S54F, GFP-M151, GFP-M24 or GFP-M196. Error bars represent standard deviation. ****p-value < 0.0001, one-way ANOVA (D) Representative DAPI (blue) images for each of the cell lines analyzed in C. Scale bars: 400 μm. (E) The number of cells invading through 8.0 μm transwell pores with a Matrigel® coating was measured for MCF10A cells, MDA-231 cells, and MDA-231 cells expressing GFP-emerin, GFP-M45A, GFP-S54F, GFP-M151, or GFP-M196. Error bars represent standard deviation. *p-value = 0.0009, **p-value = 0.0005, ****p-value < 0.0001, one-way ANOVA (F) Representative DAPI (blue) images used for E. Scale Bars: 400 μm. (G) Scratch-wound healing assay. MCF10A cells, MDA-231 cells, and MDA-231 cells expressing GFP-emerin, GFP-M45A, GFP-S54F, GFP-M151, or GFP-M196 were plated, scratched with a pipette tip, and migration of cells into the wound area was monitored for 2, 4, 12 and 24 hours. Percent healed (%) refers to the ability of cells to migrate into the wound area. Error bars represent standard deviation. (H) Representative phase images for each cell line analyzed in G. Scale bars: 400 μm.

    Article Snippet: Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624).

    Techniques: Western Blot, Expressing, Standard Deviation, Wound Healing Assay, Transferring, Migration

    (A) Normal, non-cancerous nuclei are uniformly shaped with an organized nuclear lamina. The forces exerted on the nucleus from the cytoskeleton is countered by the forces within the nucleus, resulting in no deformations. The chromatin is compacted properly with heterochromatin tethered to the periphery and euchromatin centrally localized. (B) In a metastatic cancer cell, there is disruption of nuclear lamina proteins and significantly less emerin. This results in a disorganized nuclear lamina structure. The forces exerted on the nucleus from the cytoskeleton cannot be countered by the nucleoskeleton, resulting in a smaller, deformed nucleus that can easily migrate and metastasize. (C) When emerin is added to an invasive breast cancer cell, the nucleus can now properly organize the nucleoskeleton, which causes the nucleus to increase in size and shape. These changes block intravasation from occurring. (D) When emerin mutants that fail to bind the nucleoskeleton are added to invasive breast cancer cells, the nuclei are unable to reorganize the nuclear lamina properly. This fails to alter the nuclear morphology or size, and thus these cells can still intravasate and metastasize.

    Journal: Molecular cancer research : MCR

    Article Title: Defects in Emerin-nucleoskeleton binding disrupt nuclear structure and promote breast cancer cell motility and metastasis

    doi: 10.1158/1541-7786.MCR-20-0413

    Figure Lengend Snippet: (A) Normal, non-cancerous nuclei are uniformly shaped with an organized nuclear lamina. The forces exerted on the nucleus from the cytoskeleton is countered by the forces within the nucleus, resulting in no deformations. The chromatin is compacted properly with heterochromatin tethered to the periphery and euchromatin centrally localized. (B) In a metastatic cancer cell, there is disruption of nuclear lamina proteins and significantly less emerin. This results in a disorganized nuclear lamina structure. The forces exerted on the nucleus from the cytoskeleton cannot be countered by the nucleoskeleton, resulting in a smaller, deformed nucleus that can easily migrate and metastasize. (C) When emerin is added to an invasive breast cancer cell, the nucleus can now properly organize the nucleoskeleton, which causes the nucleus to increase in size and shape. These changes block intravasation from occurring. (D) When emerin mutants that fail to bind the nucleoskeleton are added to invasive breast cancer cells, the nuclei are unable to reorganize the nuclear lamina properly. This fails to alter the nuclear morphology or size, and thus these cells can still intravasate and metastasize.

    Article Snippet: Primary antibodies used for blotting included rabbit emerin polyclonal antibody (1:5,000 dilution; Proteintech, Rosemont, IL, cat#: 10351-I-AP), mouse gamma-tubulin monoclonal antibody (1:10,000 dilution; Sigma-Aldrich, cat#: T6557, RRID:AB_532292), mouse actin monoclonal antibody (1:2,500 dilution; Sigma-Aldrich, cat#: A5441, RRID:AB_476744), rabbit H3K9me2 polyclonal antibody (1:5,000 dilution; Active Motif, cat#: 39239, RRID:AB_2793199), rabbit H3K9me3 polyclonal antibody (1:10,000 dilution; Abcam, Cambridge, UK, cat#: ab8898, RRID:AB_306848), and rabbit H3K27me3 polyclonal antibody (1:10,000 dilution; MilliporeSigma, cat#: 07–449, RRID:AB_310624).

    Techniques: Disruption, Blocking Assay